Buffalo Presbyterian Church and Cemetery is a historic Presbyterian church complex and cemetery located at 800 and 803 Sixteenth Street in Greensboro Guilford County, North Carolina. The Federal-style church sanctuary was built in 1827, and updated and expanded in 1919-1920 in the Colonial Revival style by architect Harry Barton (1876-1937). It was expanded again in 1956. Flanking the sanctuary are two-story Colonial Revival-style educational buildings linked to it by one-story arcades. The rectangular two-story Colonial Revival-style manse and garage were added to the complex in 1924. The church cemetery has burials dating back to 1775.It was listed on the National Register of Historic Places in 2002.